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Friday, April 24, 2020 | History

4 edition of Direct Methods for Solving Macromolecular Structures found in the catalog.

Direct Methods for Solving Macromolecular Structures

  • 136 Want to read
  • 27 Currently reading

Published by Springer .
Written in English

    Subjects:
  • Crystallography,
  • Science/Mathematics,
  • Macromolecules,
  • Science,
  • Structure,
  • Chemistry - Organic,
  • Physics,
  • Science / Crystallography,
  • Congresses

  • Edition Notes

    NATO Science Series C:

    The Physical Object
    FormatHardcover
    Number of Pages400
    ID Numbers
    Open LibraryOL7808603M
    ISBN 100792349490
    ISBN 109780792349495

    The term macromolecular assembly (MA) refers to massive chemical structures such as viruses and non-biologic nanoparticles, cellular organelles and membranes and ribosomes, etc. that are complex mixtures of polypeptide, polynucleotide, polysaccharide or other polymeric are generally of more than one of these types, and the mixtures are defined spatially (i.e., with regard. Hen-egg-white lysozyme. - Hen-egg-white lysozyme (PDB file 1lyz). was the first crystal structure of an enzyme (it cleaves small carbohydrates into simple sugars), used for early studies of enzyme mechanism. It contained beta sheet (antiparallel) as well as helices, and was also the first macromolecular structure to have its atomic coordinates refined (in real space). The past decade and a half have witnessed an almost complete revolution in the way that macromolecular diffraction data are recorded. The promise of diffraction data measurements essentially free of the effects of radiation damage has driven a change from older methods requiring crystalline samples to be mounted in thin glass capillaries for measurements at ambient temperatures (or so) to.


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Direct Methods for Solving Macromolecular Structures by S. Fortier Download PDF EPUB FB2

This book of the proceedings of the NATO Advanced Study Institute (ASI) on Direct Methods for Solving Macromolecular Structures was assembled from the lecturers' contributions and represents Direct Methods for Solving Macromolecular Structures book comprehensive and in-depth overview of crystallographic structure determination methods for.

About this book. Introduction. This book of the proceedings of the NATO Advanced Study Institute (ASI) on Direct Methods for Solving Macromolecular Structures was assembled from the lecturers' contributions and represents a comprehensive and in-depth overview of crystallographic structure determination methods for macromolecules.

"Proceedings of the NATO Advanced Study Institute on Direct Methods for Solving Macromolecular Structures, Erice, Italy, May June 2, " "Published in cooperation with NATO Scientific Affairs Division." Description: x, pages: illustrations ; 25 cm. Contents. Direct Methods of Solving Crystal Structures It seems that you're in USA.

We Direct Methods for Solving Macromolecular Structures book a dedicated site for Get immediate ebook access* when you order a print book Chemistry Physical Chemistry.

Nato Science Series B: Direct Methods for Macromolecular Crystallography. Pages. Get this from a library. Direct methods for solving macromolecular structures. [Suzanne Fortier; North Atlantic Treaty Organization.

Scientific Affairs Division.;] -- The first part of the book introduces the array of tools currently in use in structure determination, originating from mathematical, computational and experimental approaches.

It displays the variety. Direct Methods for Solving Macromolecular Structures. () Direct Methods, and the use of Synchrotron Radiation for Macromolecular Crystallography.

In: Fortier S. (eds) Direct Methods for Solving Macromolecular Structures. NATO ASI Series (Series C: Mathematical and Physical Sciences), vol Author: Robert M. Sweet, John M. Skinner, Thomas C. Terwilliger. Hai-Fu F. () Direct Direct Methods for Solving Macromolecular Structures book of Solving Phase Ambiguities in Macromolecular Structures.

In: Fortier S. (eds) Direct Methods for Solving Macromolecular Structures. NATO ASI Series (Series C: Mathematical and Physical Sciences), vol Cited by: 1.

Introduction to Macromolecular Crystallography, Second Edition. Author(s): the book's content flows logically from basic principles to methods, such as those for solving phase problems, interpretation of Patterson maps and the difference Fourier method, the fundamental theory of diffraction and the properties of Direct Methods for Solving Macromolecular Structures book, and applications.

Introduction to macromolecular crystallography / Alexander McPherson. properties, and the basic concepts of solving the structures of macromolecular crystals. It The only technique that allows direct visualization of protein structure at the atomic, or.

Substructure search procedures for macromolecular structures. experimental phasing of macromolecular structures involves heavy-atom soaks and the collection of two or more data sets: the diffraction intensities of the native crystal and those of the derivative(s).

The essence of direct methods. Normalized structure factors correspond to. Integration of Direct Methods With Macromolecular Crystallographic Techniques The use of traditional direct methods for solving macromolecular crystal structures or for refining phases was.

MicroED is a cryo-EM method that determines atomic resolution structures from three-dimensional protein crystals only hundreds of nanometers in thickness and in doing so promises to provide a new approach to structure determination of Direct Methods for Solving Macromolecular Structures book structures (Shi et al., ).Cited by: 7.

Ideally, one would like to solve new structures with a single native crystal. For extremely well-diffracting crystals (resolution of better than Å), direct methods can sometimes play a role in solving the structure ab initio or with the use of minor additional by: Direct-Methods-For-Solving-Macromolecular-Structures-(Nato-Science-Series-C-Dq Adobe Acrobat Reader DCDownload Adobe Acrobat Reader DC Ebook PDF:Work on documents anywhere using the Acrobat Reader mobile app Its packed with all the tools you need to convert edit.

Direct methods solved the phase problem for small molecules, and are on the way of solving the structures of medium size molecules [1] (e.g.

– atoms in the asymmetric unit). The question is then: how useful can they be for normal size or large size macromolecules?Author: C. Giacovazzo. Abstract. Multiwavelength anomalous diffraction (MAD) is the fastest growing method of structure determination in macromolecular crystallography.

At least twenty-five new structures solved with MAD were published in the past year. Many factors contribute Cited by: 4.

Large macromolecular assemblies are exciting new targets for protein crystallography. The methods used to solve these complex structures are generally the same as for more routine problems, but it is the imagination and expertise of the crystallographer in selecting and implementing a sequence of techniques that leads to a by: 7.

Mass Spectrometry-based Methods to Study Macromolecular Higher Order Structure and Interactions. Edited by Igor A. Kaltashov. select article Mass spectrometry-based methods to study macromolecular higher order structure and interactions (FPOP) as a footprinting approach to solve diverse problems in structural biology.

Bojie Zhang, Ming. However, direct methods have been used successfully to solve high-resolution structures of smaller protein molecules; for example, a trypsin inhibitor. More typically, direct methods can be and are routinely used to locate certain ‘special’ atoms, such as heavy or anomalous scatterers, in macromolecular by: This chapter describes the steps required to carry out the two-stage phasing process for proteins and illustrates these through the application of BnP program to the multiple-wavelength anomalous dispersion (MAD) data set for the selenomethionine derivative of methylmalonyl-coenzyme A epimerase from Propionibacterium shermanii.

The BnP program has two operational modes, automatic and manual. Open Library is an open, editable library catalog, building towards a web page for every book ever published.

Direct Methods of Solving Crystal Structures by Henk Schenk,Springer edition, paperback. 5 In-house macromolecular data collection 77 Mark R. Sanderson 6 Solving the phase problem using isomorphous replacement 87 Sherin S. Abdel-Meguid 7 Molecular replacement techniques for high-throughput structure determination 97 Marc Delarue 8 MAD phasing H.

Krishna Murthy 9 Application of direct methods to macromolecular structure. S Fortier (Ed.), Direct Methods for Solving Macromolecular Structures, Kluwer Academic Publishers, Netherlands (), pp. Google Scholar A brief background to real-space refinement methods and the implementation of a target that accounts for the resolution of the experimental data are by: Introduction to Macromolecular Crystallography, the book's content flows logically from basic principles to methods, such as those for solving phase problems, interpretation of Patterson maps and the difference Fourier method, the fundamental theory of diffraction and the properties of crystals, and applications in determining Cited by: Macromolecular structures diffracting to atomic resolution ( Å or beyond) are rather an exception [less than % of the entries in the PDB (Bernstein et al., ; Berman et al., )].A general ab initio phasing method should also be able to tackle lower resolution cases.

Still, a number of useful experiences can be drawn from the atomic resolution cases and exported to the lower. Introduction. Macromolecular crystallography has come a long way in the half-century since the first protein structure (of myoglobin at 6 Å resolution) [] was establishment of the Protein Data Bank (PDB) [2,3] as the single repository for crystal structures (and later structural models obtained by NMR spectroscopy, fiber diffraction, electron microscopy, and some other Cited by: ISBN: OCLC Number: Notes: "Published in cooperation with NATO Scientific Affairs Division." "Proceedings of a NATO Advanced Study Institute/18th Course of the International School of Crystallography on Direct Methods of Solving Crystal Structures, held April, in Erice, Sicily, Italy"--Title page verso.

Introduction. A key step in the determination of macromolecular crystal structures, either by direct methods or by more traditional MAD or MIR approaches, is the evaluation of the quality of an electron-density map. In applying direct methods to macromolecular crystal structure determination, statistical relationships derived from characteristics of small-molecule structures (e.g.

Sheldrick. The method of solving the structure of macromolecules with such tiny crystals has been introduced as nanocrystallography and will be the primary content of this review.

An ideal structural method for biological macromolecules would be the one that would probe a single macromolecule and provide atomistic resolution structural : Krishna P.

Khakurel, Borislav Angelov, Jakob Andreasson. Read chapter 3 MACROMOLECULAR STRUCTURES: This book describes fruitful past collaborations between the mathematical and materials sciences and indicates.

direct methods for solving linear systems and least-squares problems, and to provide an overview of the algorithms, data structures, and software available to solve these problems, so that the reader can both understand the methodsCited by: Two major methods to obtain macromolecular structures are diffraction (usually using X-rays, but also neutrons or electrons) and electron cryo-microscopy (cryo-EM 1) (Fig.

1), both of which are handled by Phenix. The following subsections describe some concepts underpinning each method for the benefit of readers who are not experts in each of Cited by: Refinement of macromolecular structures against low-resolution crystallographic data is limited by the ability of current methods to converge on a structure with realistic geometry.

This chapter casts the high-throughput automation efforts being developed to meet the needs of Structural Genomics initiatives in the framework of an optimization problem. It gives a general overview on optimization techniques with a bias specifically towards the problem of crystallographic refinement.

This picture is extended as the chapter considers model building, program flow control. • Force method and slope-deflection method can be used, with hand calculation, for solving the indeterminate structures when the degree of static or kinematical indeterminacy is small.

• In this chapter, direct stiffness method (which is also called the displacement method) will be introduced that is a modern method for structural Size: KB. Among the many different structural biology techniques, electron cryomicroscopy (cryo-EM) combined with single particle averaging techniques has recently emerged as a preferred option for solving near-atomic resolution structures of large macromolecular complexes and, is particularly effective in the case of heterogeneous samples, less amenable Cited by: 2.

Methods: We applied a histogram-based outlier score (HBOS) to six sets of cryo-EM atomic structures and five sets of X-ray atomic structures, including one derived from X-ray data with better than. There are three basic ways of solving macromolecular crystal structures: (1) the molecular replacement method, (2) direct methods, and (3) the heavy-atom method.

Stanford Libraries' official online search tool for books, media, journals, databases, government documents and more. Macromolecular crystallography: conventional and high-throughput methods in SearchWorks catalog. In applying direct methods to macromolecular crystal structure determination, statistical relationships derived from characteristics of small-molecule structures (e.g.

Sheldrick, ; Weeks et al., ; Hauptman, ) are typically used to discriminate between possible phase sets. In the MAD or MIR approaches, the crystallographer Cited by: 7. In physics, the phase problem is the problem of loss pdf information concerning the phase that can pdf when making a physical measurement.

The name comes from the field of X-ray crystallography, where the phase problem has to be solved for the determination of a structure from diffraction data. The phase problem is also met in the fields of imaging and signal processing.

Macromolecular structures diffracting to download pdf resolution ( Å or beyond) are rather an exception [less than % of the entries in the PDB (Bernstein et al., ; Berman et al., )].A general ab initio phasing method should also be able to tackle lower resolution cases.

Still, a number of useful experiences can be drawn from the atomic resolution cases and exported to the lower Cited by:   Macromolecular X-ray crystallography is routinely applied to understand biological processes at a molecular ebook. How­ever, significant ebook and effort are still required to solve and complete many of these structures because of the need for manual interpretation of complex numerical data using many software packages and the repeated use of interactive three-dimensional by: